ABSTRACT The objective of this study was to evaluate the effect of adding ground soybeans and antioxidants to diet of buffaloes on intake, ruminal microbial production, total and ruminal digestibility of nutrients, and ruminal fermentation parameters. Four crossbred buffaloes with a mean weight of 506±29 kg were distributed in a 4×4 Latin square. Four diets were tested: control; diet with ground soybeans; diet with ground soybeans and supplementation with yerba mate; and diet with ground soybeans and supplementation with yerba mate and vitamin E. The addition of ground soybeans had negative effects on intake of dry matter, organic matter, crude protein, neutral detergent fiber, and acid detergent fiber, as well as ruminal digestibility of neutral detergent fiber and total carbohydrates but did not influence total digestibility of nutrients. Yet, the ground soybeans diet increased the concentration of butyrate and microbial production in the rumen. The diet with ground soybeans supplemented with yerba mate decreased the concentration of acetate and increased the concentration of propionate and increased the efficiency of synthesis and the production of microbial protein in the rumen. There was a positive additive effect of vitamin E in the presence of yerba mate, enhancing the synthesis and production efficiency of microbial protein. Thus, the addition of ground soybeans and antioxidants to diet of buffaloes improves the efficiency of microbial protein synthesis and increases the production of butyrate, without, in general, altering the total digestibility of nutrients.

Four lactating Holstein cows were assigned to a 4 × 4 Latin square design to determine the effects of feeding sticky coffee hull (SCH) as a source of antioxidants on dairy cows fed with high PUFA diets. The treatments (on DM basis) were control diet, diet with 30 g/kg of soybean oil, diet with 30 g/kg of soybean oil and 100 g/kg of SCH, and diet with 30 g/kg of soybean oil and 150 g/kg of SCH. Inclusion of 150 g/kg of SCH decreased the crude protein digestibility. Lower values of NDF digestibility were also observed when cows were fed with 100 g/kg and 150g/kg of SCH. The digestibility of NDT was lower in the control and 150 g/kg of SCH diets. Milk production and composition did not differ among the treatments. Inclusion of SCH increased the total polyphenols and flavonoids in the milk and reducing power as well. Soybean oil and SCH supplementation increased the LDL and total cholesterol concentration in the plasma. Milk fatty acid profile was barely altered by the treatments. In conclusion, the results confirmed that SCH added up to 15% in the diet did not alter milk production, improved its stability, and incorporated antioxidants substances in the milk, improving its quality for human health.

Avaliaram-se os efeitos de fazendas, ordem de parto (OP) e mês de parição sobre a produção de leite (PL), porcentagem de gordura, de proteína, contagem de células somáticas (CCS) e nitrogênio uréico no leite (NUL) de quatro fazendas leiteiras especializadas, totalizando 120 vacas em lactação. Estudaram-se, também, os efeitos do NUL sobre a taxa de gestação (TG) e taxa de inseminação (TIA) no início da lactação. Os parâmetros produtivos e de qualidade do leite foram semelhantes entre fazendas e não sofreram influência do mês de parição. A Fazenda D apresentou a maior taxa de NUL. A OP não influenciou a NUL, % de gordura, % de proteína e CCS. Os animais com NUL entre 10,1 - 13,0 mg/dL apresentaram as maiores TIA e TG, tanto nas fases de 55 - 70 dias como após 70 dias pós-parto. Conclui-se que o NUL é um parâmetro útil para monitorar o estado nutricional e reprodutivo de vacas leiteiras.

The effects of farm, parity (PO) and month of parturition on milk production, percentage of fat and protein, somatic cell count (SCC), and milk urea nitrogen (MUN) concentration were assessed on four specialized dairy farms using 120 cows. Also, the effects of MUN on gestation rate (GR) and artificial insemination rate (AIR) in early lactation were studied. The parameters of production and milk quality were similar among farms and were not influenced by the month of parturition. Farm D presented the highest MUN concentration. Concentration of MUN, percentages of fat and protein and SCC were not influenced by PO. Cows with a value of MUN between 10.1 and 13.0 mg/dL had the highest AIR and GR between days 55 and 70 postpartum and after 70 days in milk. These data suggested that MUN concentration was a useful parameter to predict the nutritional and reproductive stages of dairy cows.